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Advanced Cell Diagnostics Inc rnascope probe irf6

( A–C ) Representative images of wholemount in situ hybridization showing dact1 , dact2 , and wnt11f2 gene expression patterns. ( A ) At 8 hpf, dact2 and wnt11f2 are highly expressed in the dorsal margin and presumptive Nieuwkoop center of the gastrulating embryo, with dact1 being weakly detected (arrowhead). In contrast to wnt11f2 , dact1 , and dact2 are expressed in the presumptive dorsal mesoderm (asterisk). ( B ) Lateral (anterior to the left of page) and anterior (dorsal side toward top of page) views of bud-stage embryos. dact2 and wnt11f2 transcripts are both detected in the tailbud (asterisk) while dact2 is additionally expressed in the axial mesoderm (arrow). dact1 gene expression is concentrated to the paraxial mesoderm and the neuroectoderm (open arrowheads). ( C ) Lateral and flat-mount views of 4 ss embryos. dact2 is expressed in the anterior neural plate and polster (P), notochord (N), paraxial and presomitic mesoderm (PM) and tailbud (TB). In contrast, dact1 is expressed in the midbrain (MB) and the paraxial and presomitic mesoderm. ( D, E ) Representative lateral (anterior to left of page) images of wholemount in situ hybridization showing dact1 and dact2 expression patterns. ( D ) At 24 hpf expression is detected in the developing head. ( E ) At 48 hpf expression is detected in the developing craniofacial structures (arrow). ( F ) Representative images of <t>RNAscope</t> in situ hybridization analysis of dact1 (white) and dact2 (yellow) and <t>irf6</t> (green) expression in transverse section of 72 hpf embryos. dact1 is expressed in the ethmoid plate (ep) and palatoquadrate (pq) orofacial cartilage, while dact2 is expressed in the oral epithelium (oe). The epithelial marker irf6 is expressed in the oe and surface epithelium (se). Dapi (blue). Scale bar: 100 μm.

Rnascope Probe Irf6, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnascope+probe+irf6/pmc11581427-17-5-9?v=Advanced+Cell+Diagnostics+Inc
Average 90 stars, based on 1 article reviews

rnascope probe irf6 - by Bioz Stars, 2026-07

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1) Product Images from "Genetic requirement of dact1/2 to regulate noncanonical Wnt signaling and calpain 8 during embryonic convergent extension and craniofacial morphogenesis"

Article Title: Genetic requirement of dact1/2 to regulate noncanonical Wnt signaling and calpain 8 during embryonic convergent extension and craniofacial morphogenesis

Journal: eLife

doi: 10.7554/eLife.91648

Figure Legend Snippet: ( A–C ) Representative images of wholemount in situ hybridization showing dact1 , dact2 , and wnt11f2 gene expression patterns. ( A ) At 8 hpf, dact2 and wnt11f2 are highly expressed in the dorsal margin and presumptive Nieuwkoop center of the gastrulating embryo, with dact1 being weakly detected (arrowhead). In contrast to wnt11f2 , dact1 , and dact2 are expressed in the presumptive dorsal mesoderm (asterisk). ( B ) Lateral (anterior to the left of page) and anterior (dorsal side toward top of page) views of bud-stage embryos. dact2 and wnt11f2 transcripts are both detected in the tailbud (asterisk) while dact2 is additionally expressed in the axial mesoderm (arrow). dact1 gene expression is concentrated to the paraxial mesoderm and the neuroectoderm (open arrowheads). ( C ) Lateral and flat-mount views of 4 ss embryos. dact2 is expressed in the anterior neural plate and polster (P), notochord (N), paraxial and presomitic mesoderm (PM) and tailbud (TB). In contrast, dact1 is expressed in the midbrain (MB) and the paraxial and presomitic mesoderm. ( D, E ) Representative lateral (anterior to left of page) images of wholemount in situ hybridization showing dact1 and dact2 expression patterns. ( D ) At 24 hpf expression is detected in the developing head. ( E ) At 48 hpf expression is detected in the developing craniofacial structures (arrow). ( F ) Representative images of RNAscope in situ hybridization analysis of dact1 (white) and dact2 (yellow) and irf6 (green) expression in transverse section of 72 hpf embryos. dact1 is expressed in the ethmoid plate (ep) and palatoquadrate (pq) orofacial cartilage, while dact2 is expressed in the oral epithelium (oe). The epithelial marker irf6 is expressed in the oe and surface epithelium (se). Dapi (blue). Scale bar: 100 μm.

Techniques Used: In Situ Hybridization, Gene Expression, Expressing, RNAscope, Marker

Figure Legend Snippet:

Techniques Used: CRISPR, Mutagenesis, Reverse Transcription, Gene Expression, Recombinant, Plasmid Preparation, RNAscope, Software

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Journal: eLife

Article Title: Genetic requirement of dact1/2 to regulate noncanonical Wnt signaling and calpain 8 during embryonic convergent extension and craniofacial morphogenesis

doi: 10.7554/eLife.91648

Figure Lengend Snippet: ( A–C ) Representative images of wholemount in situ hybridization showing dact1 , dact2 , and wnt11f2 gene expression patterns. ( A ) At 8 hpf, dact2 and wnt11f2 are highly expressed in the dorsal margin and presumptive Nieuwkoop center of the gastrulating embryo, with dact1 being weakly detected (arrowhead). In contrast to wnt11f2 , dact1 , and dact2 are expressed in the presumptive dorsal mesoderm (asterisk). ( B ) Lateral (anterior to the left of page) and anterior (dorsal side toward top of page) views of bud-stage embryos. dact2 and wnt11f2 transcripts are both detected in the tailbud (asterisk) while dact2 is additionally expressed in the axial mesoderm (arrow). dact1 gene expression is concentrated to the paraxial mesoderm and the neuroectoderm (open arrowheads). ( C ) Lateral and flat-mount views of 4 ss embryos. dact2 is expressed in the anterior neural plate and polster (P), notochord (N), paraxial and presomitic mesoderm (PM) and tailbud (TB). In contrast, dact1 is expressed in the midbrain (MB) and the paraxial and presomitic mesoderm. ( D, E ) Representative lateral (anterior to left of page) images of wholemount in situ hybridization showing dact1 and dact2 expression patterns. ( D ) At 24 hpf expression is detected in the developing head. ( E ) At 48 hpf expression is detected in the developing craniofacial structures (arrow). ( F ) Representative images of RNAscope in situ hybridization analysis of dact1 (white) and dact2 (yellow) and irf6 (green) expression in transverse section of 72 hpf embryos. dact1 is expressed in the ethmoid plate (ep) and palatoquadrate (pq) orofacial cartilage, while dact2 is expressed in the oral epithelium (oe). The epithelial marker irf6 is expressed in the oe and surface epithelium (se). Dapi (blue). Scale bar: 100 μm.

Article Snippet: Commercial assay or kit , RNAscope probe irf6 , ACDbio , ID_source:identifier 555101 , .

Techniques: In Situ Hybridization, Gene Expression, Expressing, RNAscope, Marker

Journal: eLife

Article Title: Genetic requirement of dact1/2 to regulate noncanonical Wnt signaling and calpain 8 during embryonic convergent extension and craniofacial morphogenesis

doi: 10.7554/eLife.91648

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , RNAscope probe irf6 , ACDbio , ID_source:identifier 555101 , .

Techniques: CRISPR, Mutagenesis, Reverse Transcription, Gene Expression, Recombinant, Plasmid Preparation, RNAscope, Software

Irf6 is expressed with neural crest cell markers Wnt1 and Sox10 in neural folds and neural tube during early embryogenesis. In situ hybridization of Irf6 (yellow), Wnt1 (red), and Sox10 (white) RNA transcripts. A. Coronal section of E8 mouse embryo (dorsal to top) showing the neural fold. In situ hybridization shows RNA expression domains of Irf6 , Wnt1 , and Sox10 , where Irf6 and Wnt1 transcripts are found in the same regions of the neural tube, highlighted by yellow arrow. Box indicates area of higher magnification to the right. B. Sagittal section of E9 mouse embryo (cranial to left). Box indicates a magnified portion of the neural tube. Irf6 is expressed in the neuroectoderm and overlaps with Wnt1 and Sox10 expression (yellow arrows). C. Sagittal section of E9 mouse embryo (cranial to left). Box indicates a magnified portion of frontonasal prominence (FNP). Irf6 is expressed in the FNP mesenchyme, along with the migratory NCC marker Sox10 . D. Coronal section of E13.5 embryo (dorsal to top). Box indicates higher magnification of palate shelf epithelium and mesenchyme. Irf6 is highly expressed in the basal epithelium and periderm and the palate mesenchyme (yellow arrow). Blue is dapi. Scale: 100 uM.

Journal: bioRxiv

Article Title: Neural crest and periderm-specific requirements of Irf6 during neural tube and craniofacial development

doi: 10.1101/2024.06.11.598425

Figure Lengend Snippet: Irf6 is expressed with neural crest cell markers Wnt1 and Sox10 in neural folds and neural tube during early embryogenesis. In situ hybridization of Irf6 (yellow), Wnt1 (red), and Sox10 (white) RNA transcripts. A. Coronal section of E8 mouse embryo (dorsal to top) showing the neural fold. In situ hybridization shows RNA expression domains of Irf6 , Wnt1 , and Sox10 , where Irf6 and Wnt1 transcripts are found in the same regions of the neural tube, highlighted by yellow arrow. Box indicates area of higher magnification to the right. B. Sagittal section of E9 mouse embryo (cranial to left). Box indicates a magnified portion of the neural tube. Irf6 is expressed in the neuroectoderm and overlaps with Wnt1 and Sox10 expression (yellow arrows). C. Sagittal section of E9 mouse embryo (cranial to left). Box indicates a magnified portion of frontonasal prominence (FNP). Irf6 is expressed in the FNP mesenchyme, along with the migratory NCC marker Sox10 . D. Coronal section of E13.5 embryo (dorsal to top). Box indicates higher magnification of palate shelf epithelium and mesenchyme. Irf6 is highly expressed in the basal epithelium and periderm and the palate mesenchyme (yellow arrow). Blue is dapi. Scale: 100 uM.

Article Snippet: RNAscope probes for mouse Irf6 , Wnt1, and Sox10 were designed and manufactured by Advanced Cell Diagnostics.

Techniques: In Situ Hybridization, RNA Expression, Expressing, Marker

Generation and validation of a conditional Irf6 null mouse model. A. Schematic of gene targeting strategy. Introns flanking Irf6 exons 3 and 4 were targeted for CRISPR-Cas9-directed homologous recombination with each donor ssDNA containing loxP sequences (green triangles). Insertion of loxP sites into Irf6 was confirmed by PCR. B . and Sanger sequencing. C. Cre-mediated recombination was validated using the ubiquitous Cre expressing lines CMV -Cre and EIIa -Cre. CMV -Cre + ; Irf6 fl/fl and EIIa -Cre + ; Irf6 fl/fl mice phenocopied the Irf6 global KO while Cre - ;Irf6 fl/fl and Cre + ; Irf6 wt/wt littermates were normal. D. Hematoxylin and Eosin staining of coronal sections of E15 CMV -Cre or EIIa -Cre knockout embryos and littermate controls. Top row is a relatively anterior section while the bottom row is relatively posterior. CMV -Cre and EIIa -Cre Irf6 KO embryos phenocopy the dysmorphic alveolar bone and the cleft palate with oral adhesions of the total Irf6 knockout mouse (arrows).

Journal: bioRxiv

Article Title: Neural crest and periderm-specific requirements of Irf6 during neural tube and craniofacial development

doi: 10.1101/2024.06.11.598425

Figure Lengend Snippet: Generation and validation of a conditional Irf6 null mouse model. A. Schematic of gene targeting strategy. Introns flanking Irf6 exons 3 and 4 were targeted for CRISPR-Cas9-directed homologous recombination with each donor ssDNA containing loxP sequences (green triangles). Insertion of loxP sites into Irf6 was confirmed by PCR. B . and Sanger sequencing. C. Cre-mediated recombination was validated using the ubiquitous Cre expressing lines CMV -Cre and EIIa -Cre. CMV -Cre + ; Irf6 fl/fl and EIIa -Cre + ; Irf6 fl/fl mice phenocopied the Irf6 global KO while Cre - ;Irf6 fl/fl and Cre + ; Irf6 wt/wt littermates were normal. D. Hematoxylin and Eosin staining of coronal sections of E15 CMV -Cre or EIIa -Cre knockout embryos and littermate controls. Top row is a relatively anterior section while the bottom row is relatively posterior. CMV -Cre and EIIa -Cre Irf6 KO embryos phenocopy the dysmorphic alveolar bone and the cleft palate with oral adhesions of the total Irf6 knockout mouse (arrows).

Article Snippet: RNAscope probes for mouse Irf6 , Wnt1, and Sox10 were designed and manufactured by Advanced Cell Diagnostics.

Techniques: Biomarker Discovery, CRISPR, Homologous Recombination, Sequencing, Expressing, Staining, Knock-Out

Wnt1 -Cre-dependent Irf6 ablation causes cranial defects. A. Representative images of littermate control and Wnt1 -Cre, Irf6 cKO pups at P0. At parturition, Wnt1 -Cre + ; Irf6 fl/fl cKO mice display midline lesions of varying penetrance (arrow). B. Representative images of littermate control and Wnt1 -Cre + ; Irf6 fl/fl cKO pups at P6. As the mouse neonate develops, these frontal lesions resolve but remain evident with deficient or delayed fur growth (arrow). C. Hematoxylin and eosin staining of coronal sections through the palate of E16 Wnt1 -Cre + ; Irf6 fl/fl cKO and littermate control embryos shows normal development (arrow). D. Hematoxylin and eosin staining of coronal sections through the nasal and frontal bones of Wnt1 -Cre + ; Irf6 fl/fl cKO and littermate control. Sections move anterior to posterior from left to right. Bone tissue is indicated with arrows. Wnt1 -Cre + ; Irf6 fl/fl cKO mice have a lack of cranial bone development and suture formation at the midline (bone tissue indicated by arrows). Scale: 100 µM.

Journal: bioRxiv

Article Title: Neural crest and periderm-specific requirements of Irf6 during neural tube and craniofacial development

doi: 10.1101/2024.06.11.598425

Figure Lengend Snippet: Wnt1 -Cre-dependent Irf6 ablation causes cranial defects. A. Representative images of littermate control and Wnt1 -Cre, Irf6 cKO pups at P0. At parturition, Wnt1 -Cre + ; Irf6 fl/fl cKO mice display midline lesions of varying penetrance (arrow). B. Representative images of littermate control and Wnt1 -Cre + ; Irf6 fl/fl cKO pups at P6. As the mouse neonate develops, these frontal lesions resolve but remain evident with deficient or delayed fur growth (arrow). C. Hematoxylin and eosin staining of coronal sections through the palate of E16 Wnt1 -Cre + ; Irf6 fl/fl cKO and littermate control embryos shows normal development (arrow). D. Hematoxylin and eosin staining of coronal sections through the nasal and frontal bones of Wnt1 -Cre + ; Irf6 fl/fl cKO and littermate control. Sections move anterior to posterior from left to right. Bone tissue is indicated with arrows. Wnt1 -Cre + ; Irf6 fl/fl cKO mice have a lack of cranial bone development and suture formation at the midline (bone tissue indicated by arrows). Scale: 100 µM.

Article Snippet: RNAscope probes for mouse Irf6 , Wnt1, and Sox10 were designed and manufactured by Advanced Cell Diagnostics.

Techniques: Control, Staining

Cranial bone development is impaired in Wnt1- Cre Irf6 cKO mice. A. Representative microCT reconstructions of P10 Wnt1 -Cre + ; Irf6 fl/fl cKO mice and littermate sex-matched controls. Wnt1 -Cre + ; Irf6 fl/fl cKO mice have decreased formation or mineralization of the cranial bones at the midline with variable penetrance (arrows). Scale: 1 mm. B. MicroCT reconstructions were utilized for cranial bone measurements. The space between the left and right frontal bones of Wnt1 -Cre + ; Irf6 fl/fl cKO mice was significantly wider than controls (L1-R1, *p<0.05) and the frontal bones tended to have decreased total length (length 1-2). Maxilla of Wnt1 -Cre + ; Irf6 fl/fl cKO mice tended to be smaller (lower length and width measurements) and the frontal bone of Wnt1 -Cre + ; Irf6 fl/fl cKO mice tended to be shorter, however, these differences were not significantly different. N=4.

Journal: bioRxiv

Article Title: Neural crest and periderm-specific requirements of Irf6 during neural tube and craniofacial development

doi: 10.1101/2024.06.11.598425

Figure Lengend Snippet: Cranial bone development is impaired in Wnt1- Cre Irf6 cKO mice. A. Representative microCT reconstructions of P10 Wnt1 -Cre + ; Irf6 fl/fl cKO mice and littermate sex-matched controls. Wnt1 -Cre + ; Irf6 fl/fl cKO mice have decreased formation or mineralization of the cranial bones at the midline with variable penetrance (arrows). Scale: 1 mm. B. MicroCT reconstructions were utilized for cranial bone measurements. The space between the left and right frontal bones of Wnt1 -Cre + ; Irf6 fl/fl cKO mice was significantly wider than controls (L1-R1, *p<0.05) and the frontal bones tended to have decreased total length (length 1-2). Maxilla of Wnt1 -Cre + ; Irf6 fl/fl cKO mice tended to be smaller (lower length and width measurements) and the frontal bone of Wnt1 -Cre + ; Irf6 fl/fl cKO mice tended to be shorter, however, these differences were not significantly different. N=4.

Article Snippet: RNAscope probes for mouse Irf6 , Wnt1, and Sox10 were designed and manufactured by Advanced Cell Diagnostics.

Techniques:

Irf6 ablation in the neuroectoderm and neural crest changes Wnt1 expression domains within the neural folds. A. RNAscope in situ hybridization of transverse sections of Wnt1 -Cre + ; Irf6 fl/fl cKO and littermate control E8 embryos. Rows represent 2 individuals of each genotype. Whereas Wnt1 expression (red) is localized to the caudal-dorsal neural folds in the control embryos, Wnt1 expression in Wnt1 -Cre + ; Irf6 fl/fl cKO embryos is displaced laterally (arrows). Blue is dapi. Scale: 100 µM

Journal: bioRxiv

Article Title: Neural crest and periderm-specific requirements of Irf6 during neural tube and craniofacial development

doi: 10.1101/2024.06.11.598425

Figure Lengend Snippet: Irf6 ablation in the neuroectoderm and neural crest changes Wnt1 expression domains within the neural folds. A. RNAscope in situ hybridization of transverse sections of Wnt1 -Cre + ; Irf6 fl/fl cKO and littermate control E8 embryos. Rows represent 2 individuals of each genotype. Whereas Wnt1 expression (red) is localized to the caudal-dorsal neural folds in the control embryos, Wnt1 expression in Wnt1 -Cre + ; Irf6 fl/fl cKO embryos is displaced laterally (arrows). Blue is dapi. Scale: 100 µM

Article Snippet: RNAscope probes for mouse Irf6 , Wnt1, and Sox10 were designed and manufactured by Advanced Cell Diagnostics.

Techniques: Expressing, RNAscope, In Situ Hybridization, Control

Periderm-specific ablation of Irf6 results in a comparable but mild form of the global Irf6 KO phenotype. Krt6ai -Cre + ; Irf6 fl/fl and littermate control neonates were collected at P1. A. Lateral and caudal representation of neonates comparing control Krt6ai -Cre - ; Irf6 fl/fl with Krt6ai -Cre + ; Irf6 fl/fl cKO. B. Krt6ai -Cre - ; Irf6 fl/fl exhibit normal skin and digits; however Krt6ai -Cre + ; Irf6 fl/fl reveal abnormal skin and fused digits phenotype. Scale: 500 µM. C. Hematoxylin and Eosin staining of coronal sections through vomeronasal and primary palate of neonates. Krt6ai -Cre - ; Irf6 fl/fl mice show normal septum and palate. Krt6ai -Cre + ; Irf6 fl/fl mice reveal abnormal septum and adhesions of the tongue.

Journal: bioRxiv

Article Title: Neural crest and periderm-specific requirements of Irf6 during neural tube and craniofacial development

doi: 10.1101/2024.06.11.598425

Figure Lengend Snippet: Periderm-specific ablation of Irf6 results in a comparable but mild form of the global Irf6 KO phenotype. Krt6ai -Cre + ; Irf6 fl/fl and littermate control neonates were collected at P1. A. Lateral and caudal representation of neonates comparing control Krt6ai -Cre - ; Irf6 fl/fl with Krt6ai -Cre + ; Irf6 fl/fl cKO. B. Krt6ai -Cre - ; Irf6 fl/fl exhibit normal skin and digits; however Krt6ai -Cre + ; Irf6 fl/fl reveal abnormal skin and fused digits phenotype. Scale: 500 µM. C. Hematoxylin and Eosin staining of coronal sections through vomeronasal and primary palate of neonates. Krt6ai -Cre - ; Irf6 fl/fl mice show normal septum and palate. Krt6ai -Cre + ; Irf6 fl/fl mice reveal abnormal septum and adhesions of the tongue.

Article Snippet: RNAscope probes for mouse Irf6 , Wnt1, and Sox10 were designed and manufactured by Advanced Cell Diagnostics.

Techniques: Control, Staining

Crect -driven Irf6 ablation recapitulates the global Irf6 KO phenotype. A. Representative images of littermate control and Crect + - Irf6 fl/fl cKO pups at approximately E17. Crect + - Irf6 fl/fl pups exhibit “cocooning” taught skin, abnormal and shortened limbs, and an umbilical hernia that has been described for the Irf6 global KO. B. Hematoxylin and eosin staining of coronal sections of approximately E17 Crect + - Irf6 fl/fl pup and littermate control. Crect + - Irf6 fl/fl cKO pups exhibit severe oral adhesions and cleft palate similar to the global Irf6 KO mouse. Scale: 100 µM.

Journal: bioRxiv

Article Title: Neural crest and periderm-specific requirements of Irf6 during neural tube and craniofacial development

doi: 10.1101/2024.06.11.598425

Figure Lengend Snippet: Crect -driven Irf6 ablation recapitulates the global Irf6 KO phenotype. A. Representative images of littermate control and Crect + - Irf6 fl/fl cKO pups at approximately E17. Crect + - Irf6 fl/fl pups exhibit “cocooning” taught skin, abnormal and shortened limbs, and an umbilical hernia that has been described for the Irf6 global KO. B. Hematoxylin and eosin staining of coronal sections of approximately E17 Crect + - Irf6 fl/fl pup and littermate control. Crect + - Irf6 fl/fl cKO pups exhibit severe oral adhesions and cleft palate similar to the global Irf6 KO mouse. Scale: 100 µM.

Article Snippet: RNAscope probes for mouse Irf6 , Wnt1, and Sox10 were designed and manufactured by Advanced Cell Diagnostics.

Techniques: Control, Staining

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1) Product Images from "Genetic requirement of dact1/2 to regulate noncanonical Wnt signaling and calpain 8 during embryonic convergent extension and craniofacial morphogenesis"

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